The Department CIBIO Advanced Imaging Facility provides access to advanced biomedical imaging technology including state-of-the-art light microscopes, as well as image analysis software packages.

The Facility equipment consists of:

  • Zeiss Axio Observer Z1 equipped with Colibri 1, ApoTome 1 and Cell Observer modules
  • Zeiss Axio Imager M2 equipped with MBF motorised stage for stereology
  • Leica DM6000 B equipped with Leica LMD6500 Laser Micro-dissection System
  • Till Photonics iMIC digital microscope equipped with multicolor fluorescence, structured illumination and TIRF iMIC packages
  • Nikon Eclipse 90i fluorescence microscope
  • Leica MZ 16F fluorescence stereomicroscope
  • TILL Photonics iMIC digital microscope equipped with a multicolor fluorescence iMIC package for general fluorescence applications
  • Leica TCS SP5 confocal microscope

The available applications are:

  • Wide-field fluorescence microscopy
  • Trasmitted light (Bright-field, Phase Contrast, Differential Interference Contrast and Dark-field) microscopy
  • Fluorescence Lifetime Imaging Microscopy
  • Automated wide-field imaging
  • Environmental control for in-vivo imaging
  • 3D imaging
  • Laser micro-dissection of tissues at single-cell level
  • Confocal microscopy
  • Image processing and analysis

The services offered by the AICF Staff are:

  • Trainings for members and guests of the Institute for the autonomous use of the microscope
  • Shadowing of non-autonomous users
  • Services for internal and external Cibio users on not bookable instruments
  • Data analysis
  • Technical support in experimental design, microscope purchase and configuration

The post-processing software available in the Facility are:

  • The open-source software ImageJ and Fiji directly accessible by any user with its computer or by AICF working station
  • SVI Huygens software (via the web interface Huygens Remote Manager v.3.0.3 and VPN connection) for deconvolution analysis and Huygens Essential for distilling PSF
  • MatLab

The CIBIO AICF is part of the LabSSAH lab, a partnership among Department CIBIO, the Bruno Kessler Foundation (FBK) and the CNR-Institute of Biophysics Unit at Trento (IBF-TN).