Overview | Research directions | Group members | Collaborations | Selected Publications | Funding

Overview

Replicative immortality is one of the hallmarks of cancer and is achieved by cancer cells through activation of telomere maintenance mechanisms. Most human cancers rely on expression of the reverse transcriptase telomerase to maintain telomere length. Cancers that do not express telomerase utilize alternative mechanisms of telomere elongation, known as ALT. Cancer cells can also trigger a telomerase-to-ALT transition to acquire resistance to telomerase-targeting therapies. One of the hallmarks of ALT is increased telomere transcription and induced expression of the telomeric noncoding RNAs TERRA which have been proposed to sustain ALT mechanisms through formation of RNA:DNA hybrids at telomeres. Furthermore, TERRA interacts with telomerase as well as ALT-relevant proteins, including ATRX, and TERRA transcripts can promote heterochromatin formation at telomeres. Thus, TERRA transcripts are critical regulators of telomere maintenance of cancer cells. 

Research directions

We are interested in understanding the function and regulation of TERRA in human cancer cells. To this aim, we have developed a live-cell imaging assay to visualize and study endogenous TERRA transcripts expressed from a single telomere in living human cancer cells. By combining live-cell imaging with biochemical and molecular biology approaches we aim to define the dynamics of TERRA expression and localization at telomeres and characterize the role of TERRA in telomere maintenance of human cancer cells.

Group members

  • Emilio Cusanelli, PI 
  • Katarina Jurikova, Postdoc
  • Nicole Bettin, PhD Student
  • Valeria Savoca, PhD Student
  • Caterina Manzato, Trainee
  • Beatrice Zita Passerini, Master Student
  • Laura Avino, Master Student

We are currently looking for a highly motivated postdoc to work on a new project to study the function of TERRA in cancer cells. For information please contact directly: emilio.cusanelli [at] unitn.it

Collaborations

  • Pascal Chartrand, University of Montreal, Canada
  • Verena Jantsch, University of Vienna, Austria
  • Maria Caterina Mione, University of Trento
  • Paolo Macchi, University of Trento
  • Luciano Conti, University of Trento

Selected publications

Bettin N, Oss Pegorar C, Cusanelli E. The emerging roles of TERRA in telomere maintenance and genome stability. Cells, 2019, 8(3), 246.

Avogaro L, Oss Pegorar C, Bettin N, Cusanelli E. Generation of cancer cell clones to visualize telomeric repeat-containing RNA TERRA expressed from a single telomere in living cells. Journal of Visualized Experiments, 2019, (143): e58790.

Avogaro L, Querido E, Dalachi M, Jantsch M, Chartrand P, Cusanelli E. Live-cell imaging reveals the dynamics and function of single-telomere TERRA molecules in cancer cells. RNA Biology, 2018, 15(6):787-796.

Perez-Romero CA, Lalonde M, Chartrand P, Cusanelli E. Induction and relocalization of telomeric repeat-containing RNAs during diauxic shift in budding yeast. Current Genetics, 2018, 64(5):1117-1127.

Moradi-Fard S, Sarthi J, Tittel-Elmer M, Lalonde M, Cusanelli E, Chartrand P, Cobb J. Smc5/6 is a telomere-associated complex that regulates Sir4 binding and TPE. PLOS Genetics, 2016, 12(8): e1006268.

Cusanelli E* and Chartrand P. Telomeric repeat-containing RNA TERRA: a noncoding RNA connecting telomere biology to genome integrity. Frontiers in Genetics, 2015, 6: 143.                                * Corresponding author.

Cusanelli E and Chartrand P. Telomeric non-coding RNA TERRA in telomere biology. Wiley Interd. Rev. RNA, 2014, 5(3): 407-419.

Cusanelli E, Perez Romero CA, Chartrand P. Telomeric non-coding RNA TERRA is induced by telomere shortening to nucleate telomerase molecules at short telomeres. Molecular Cell, 2013, 51(6): 780-791. Highlighted by Faculty of 1000

Gallardo F*, Laterreur N*, Cusanelli E, Ouenzar F, Querido E, Wellinger RJ, Chartrand P. Live cell imaging of telomerase RNA dynamics reveals cell cycle-dependent clustering of telomerase at elongating telomeres. Molecular Cell, 2011, 44(5): 819–827.

* Equally contributed.

2020, My First AIRC Grant

2016, Rita Levi Montalcini program, (MIUR)