The Department CIBIO Cell Technology Facility offers resources and expertise to improve the understanding of life science through advanced cell technologies through the generation of in vitro tools able to model development and diseases.
The Cell Technology Facility mission is to integrate the multidisciplinary research interests of CIBIO scientists and facilitate the access to stem cell and genome editing technologies. It provides customized services, that include scientific support, assistance for project design, training activities, data analysis and experimental execution.
The Facility services include:
- Somatic cell reprogramming finalized to the obtainment of customized induced Pluripotent Stem Cells (iPSCs), eventually differentiable in all the mature cell types.
- Development of protocols for stem cell differentiation in two- or three-dimensional culturing conditions.
- Set-up of cancer stem cells cultures.
- Genome editing platforms, in order to produce specifically engineered cell lines, carrying desired deletions, insertions, mutations or tags.
- Cell line metabolic phenotyping using the Seahorse XFp Analyser.
- Engineering of hard to transfect cell lines by infection, nucleofection and electroporation.
- In vitro/ex vivo extracellular electrophysiology by BioCAM X Multielectrode Array.
- Mycoplasma detection in cell culture.
- Production of R-Spondin1, WNT3A, Noggin/R-Spodin3, WNT3A conditioned-media.