Extracellular vesicles (EVs) are lipid-composed particles, in the range of nanometers, secreted from the cells. EVs entrap several molecules, such as different kind of lipids, RNA, proteins, and metabolites that can be produced to influence recipient cells, therefore mediating a sort of communication. Cells from different tissues release billions of circulating EVs that can be retrieved from liquid biopsies.
We study EVs with three major goals:
- Exploit them to develop novel precision medicine tools: We sought to apply high-throughput procedures to isolate EVs from cell culture media or biological fluids (blood, urine, CSF, saliva) and develop quantitative approaches to probe markers mirroring the cell heterogeneity that characterizes complex disorders;
- Apply quantitative technologies to understand the functional effect of the horizontal material transfer to recipient cells: Mostly focused at RNA and protein levels and on the discovery of modulators of their sorting into EVs;
- Build novel ex-vivo therapeutic particles: EVs expose specific transmembrane proteins found in the cells that have originated them. Several tools for EV engineering and production can be explored for the design of a new generation of vectors with a defined bio-distribution and promising anti-cancer effects.
- Michela Notarangelo, Postdoc
- Elena Gurrieri, PhD Student
- Fabrizio Fabbiano, PhD Student
- Jessica Corsi, PhD Student
- Caterina Trevisan, Post-laurea
- Elena Elez, VHIO, Spain
- Stefan Lehr, DDZ, Germany
- Tero Aittokallio, FIMM, Finland
- Robin Ristl, MUW, Austria
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