Our mission is to facilitate the development of screening programs and to offer an interactive and open environment to researchers willing to approach chemical and functional biology, or image-based cytometry. We also provide other tools for validating High Throughput Technologies findings, such as DNA/RNA analysis.
The facility is continuously being upgraded in order to support researchers with cutting-edge technologies.
We offer our service and expertise to both academic and industrial partners.
High Throughput Screening (HTS) and High Content Analysis (HCA)
For a single drug or drug combination testing and for library screenings, we offer a wide choice of ready assays and a great flexibility for new assay development.
Cell-based assays
- viability/cytotoxicity
- proliferation
- nascent RNA and proteins
- apoptosis
- genotoxicity
- migration
- invasion
- neurite outgrowth
- mitochondrial status
- reporter-based
- label-free
- metabolic activity
- others
Biochemical assays
- fluorescence
- luminescence
- TR-FRET
- Alphascreen™
A long and broad experience on HCS (PerkinElmer Operetta® High Content Imaging System and Molecular Device Image Xpress® Micro Confocal) allows us to handle:
- 2D cellular models
- 3D cultures (i.e. spheroids, organoids)
- whole organisms (i.e. zebrafish)
- target-based investigations
- multi-parameter phenotypic profiling
- end-point or kinetic mode
The automated liquid handlers (Beckman Coulter Echo 650,Tecan EVO200 and Biotek EL460) allow to perform high throughput screenings with our libraries (small molecules and siRNA) or with libraries provided by the customer.
HTS data processing is performed using open-source software platforms (R, KNIME).
See download box for Equipment and Libraries.
Nucleic Acid Analysis
We provide nucleic acid sample analysis services, from simple quantification (Thermo Fisher NanoDrop™ 2000, Qubit™ 2.0 Fluorometer ) to quality check (Agilent Bioanalyzer 2100).
We support quantitative analysis of DNA/RNA targets with real-time PCR (96 and 384 well format) for relative quantification and digital droplet PCR (Bio-Rad QX200™ Droplet Digital PCR System, ddPCR™) for absolute highly sensitive quantification.
